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An ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV) based on the specific solubility of the viral surface glycoprotein

Viral proteins of porcine epidemic diarrhoea virus (PEDV) were extracted from the cytoplasm of infected Vero cells using hypotonic conditions and a non-ionic detergent. Both the pH and the NaCl concentration of the extraction buffer were varied in attempts to increase the solubility of the virion sp...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Vet Microbiol
Main Authors: Knuchel, M., Ackermann, M., Müller, H.K., Kihm, U.
Format: Artigo
Jezik:Inglês
Izdano: Published by Elsevier B.V. 1992
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7117511/
https://ncbi.nlm.nih.gov/pubmed/1441196
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0378-1135(92)90100-8
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