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Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120
Infectious bronchitis virus (IBV) strain H120 was successfully rescued as infectious clone by reverse genetics. Thirteen 1.5–2.8 kb fragments contiguously spanning the virus genome were amplified and cloned into pMD19-T. Transcription grade complete length cDNA was acquired by a modified “No See’m”...
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| Publicado no: | Vet Microbiol |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier B.V.
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7117355/ https://ncbi.nlm.nih.gov/pubmed/22999521 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vetmic.2012.08.013 |
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