Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120

Infectious bronchitis virus (IBV) strain H120 was successfully rescued as infectious clone by reverse genetics. Thirteen 1.5–2.8 kb fragments contiguously spanning the virus genome were amplified and cloned into pMD19-T. Transcription grade complete length cDNA was acquired by a modified “No See’m”...

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Publicat a:Vet Microbiol
Autors principals: Zhou, Ying Shun, Zhang, Yi, Wang, Hong Ning, Fan, Wen Qiao, Yang, Xin, Zhang, An Yun, Zeng, Fan Ya, Zhang, Zhi Kun, Cao, Hai Peng, Zeng, Cheng
Format: Artigo
Idioma:Inglês
Publicat: Elsevier B.V. 2013
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7117355/
https://ncbi.nlm.nih.gov/pubmed/22999521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vetmic.2012.08.013
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