Expression and functional analysis of porcine aminopeptidase N produced in prokaryotic expression system

Porcine aminopeptidase N (pAPN) is a cellular membrane protein and a functional receptor for porcine coronaviruses. Here, we describe the heterologous expression of pAPN without signal peptide in BL21(DE3)pLysS host cells. The Escherichia coli (E. coli) harboring the recombinant construct was effici...

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Bibliographic Details
Published in:J Biotechnol
Main Authors: Liu, Boqi, Li, Guangxing, Sui, Xiuwen, Yin, Jiechao, Wang, Heng, Ren, Xiaofeng
Format: Artigo
Language:Inglês
Published: Elsevier B.V. 2009
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7114256/
https://ncbi.nlm.nih.gov/pubmed/19428736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbiotec.2009.02.005
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