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Development of a one-step real-time quantitative PCR assay based on primer-probe energy transfer for the detection of porcine reproductive and respiratory syndrome virus

A one-step real-time RT-PCR method has been developed for the simultaneous detection of both genotypes of porcine reproductive and respiratory syndrome virus (PRRSV). The assay is based on primer-probe energy transfer, and the most important advantage of this is the relative tolerance towards mutati...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Balka, Gyula, Hornyák, Ákos, Bálint, Ádám, Benyeda, Zsófia, Rusvai, Miklós
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier B.V. 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112897/
https://ncbi.nlm.nih.gov/pubmed/19200443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.01.014
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