A human immunodeficiency virus type 1 protease biosensor assay using bioluminescence resonance energy transfer

A sensitive reporter assay to measure human immunodeficiency virus type 1 (HIV-1) protease (PR) activity is described in this manuscript. This assay measures PR activity as a function of the resonance energy transfer (RET) between a donour molecule [humanized sea pansy Renilla reniformis luciferase...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Hu, Kimberly, Clément, Jean-Francois, Abrahamyan, Levon, Strebel, Klaus, Bouvier, Michel, Kleiman, Lawrence, Mouland, Andrew J.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier B.V. 2005
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112859/
https://ncbi.nlm.nih.gov/pubmed/15951029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2005.04.012
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