A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67 × 10(0) copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detect...

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Publicado en:J Virol Methods
Autores principales: Fan, Qing, Xie, Zhixun, Xie, Liji, Liu, Jiabo, Pang, Yaoshan, Deng, Xianwen, Xie, Zhiqin, Peng, Yi, Wang, Xiuqing
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier B.V. Published by Elsevier B.V. 2012
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112856/
https://ncbi.nlm.nih.gov/pubmed/22947692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2012.08.007
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