Development of a one step real time RT-PCR assay to detect and quantify Dugbe virus

A one-step real time quantitative RT-PCR (qRT-PCR) assay was developed to detect all published Dugbe virus (DUGV) genomes of the Nairovirus genus. Primers and probes were designed to detect specific sequences on the most conserved regions of the S segment. The limit of detection of the assay was 10...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Rodrigues, R., Telles, J.-N., Essere, K., Ducournau, C., Roqueplo, C., Levieuge, A., Davoust, B., Parola, P., Paranhos-Baccalà, G., Peyrefitte, C.N.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier B.V. 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112838/
https://ncbi.nlm.nih.gov/pubmed/21703306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2011.06.003
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