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Development of a real-time TaqMan(®) RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation
A sensitive and specific method for the diagnosis of infectious bronchitis virus (IBV) is of great importance. In this study the development of a real-time TaqMan(®) RT-PCR targeting the highly conserved nucleocapsid (N) gene of IBV and including an internal PCR control is described. The assay was s...
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| Publicado no: | J Virol Methods |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier B.V.
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7112792/ https://ncbi.nlm.nih.gov/pubmed/19781572 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.09.014 |
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