A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification

An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. Clinical samples were collected from 120 patients diagnosed...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Res Commun
Main Authors: Lau, Lok Ting, Fung, Yin-Wan Wendy, Wong, Freda Pui-Fan, Lin, Selma Sau-Wah, Wang, Chen Ran, Li, Hui Li, Dillon, Natalie, Collins, Richard A, Tam, John Siu-Lun, Chan, Paul K.S, Wang, Chen G, Yu, Albert Cheung-Hoi
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Inc. 2003
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7111096/
https://ncbi.nlm.nih.gov/pubmed/14652014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2003.11.064
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