A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis

Cell-free protein synthesis is a useful method for preparing proteins for functional or structural analyses. However, batch-to-batch variability with regard to protein synthesis activity remains a problem for large-scale production of cell extract in the laboratory. To address this issue, we have de...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Biochem
Main Authors: Katsura, Kazushige, Matsuda, Takayoshi, Tomabechi, Yuri, Yonemochi, Mayumi, Hanada, Kazuharu, Ohsawa, Noboru, Sakamoto, Kensaku, Takemoto, Chie, Shirouzu, Mikako
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2017
Teme:
Regular Papers
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7109869/
https://ncbi.nlm.nih.gov/pubmed/28992119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jb/mvx039
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