Efficient Generation of CRISPR/Cas9-Mediated Homozygous/Biallelic Medicago truncatula Mutants Using a Hairy Root System

In the process of acquiring mutants mediated by CRISPR/Cas9, plantlets are often regenerated from both mutated and non-mutated cells in a random manner, which increase the odds of chimeric mutated plant. In general, it’s necessary to infect more explants or grow to next generation for the need of ge...

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Bibliographic Details
Published in:Front Plant Sci
Main Authors: Zhang, Hailing, Cao, Yingping, Zhang, Huan, Xu, Yue, Zhou, Chuanen, Liu, Wenwen, Zhu, Ruifen, Shang, Chen, Li, Jikai, Shen, Zhongbao, Guo, Siyi, Hu, Zhubing, Fu, Chunxiang, Sun, Dequan
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2020
Subjects:
Plant Science
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7105802/
https://ncbi.nlm.nih.gov/pubmed/32265954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2020.00294
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