Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology

A type of chromosome-free cell called SimCells (simple cells) has been generated from Escherichia coli, Pseudomonas putida, and Ralstonia eutropha. The removal of the native chromosomes of these bacteria was achieved by double-stranded breaks made by heterologous I-CeuI endonuclease and the degradat...

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Dades bibliogràfiques
Publicat a:Proc Natl Acad Sci U S A
Autors principals: Fan, Catherine, Davison, Paul A., Habgood, Robert, Zeng, Hong, Decker, Christoph M., Gesell Salazar, Manuela, Lueangwattanapong, Khemmathin, Townley, Helen E., Yang, Aidong, Thompson, Ian P., Ye, Hua, Cui, Zhanfeng, Schmidt, Frank, Hunter, C. Neil, Huang, Wei E.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2020
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7104398/
https://ncbi.nlm.nih.gov/pubmed/32144140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1918859117
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