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A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity

OBJECTIVES: The construction and validation of a set of Yarrowia lipolytica CRISPR/Cas9 vectors containing six different markers that allows virtually any genetic background to be edited, including those of wild-type strains. RESULTS: Using the Golden Gate method, we assembled a set of six CRISPR/Ca...

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Detalhes bibliográficos
Publicado no:Biotechnol Lett
Main Authors: Larroude, Macarena, Trabelsi, Heykel, Nicaud, Jean-Marc, Rossignol, Tristan
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7101291/
https://ncbi.nlm.nih.gov/pubmed/31974649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-020-02805-4
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