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Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression in Chlamydomonas reinhardtii via a CRISPR-Cas9-Mediated Knock-in Method

Chlamydomonas reinhardtii is being transformed from a model organism to an industrial organism for the production of pigments, fatty acids, and pharmaceuticals. Genetic modification has been used to increase the economic value of C. reinhardtii. However, low gene-editing efficiency and position-effe...

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Bibliografiske detaljer
Udgivet i:Front Plant Sci
Main Authors: Kim, Jongrae, Lee, Sangmuk, Baek, Kwangryul, Jin, EonSeon
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7099044/
https://ncbi.nlm.nih.gov/pubmed/32265959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2020.00306
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