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Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression in Chlamydomonas reinhardtii via a CRISPR-Cas9-Mediated Knock-in Method

Chlamydomonas reinhardtii is being transformed from a model organism to an industrial organism for the production of pigments, fatty acids, and pharmaceuticals. Genetic modification has been used to increase the economic value of C. reinhardtii. However, low gene-editing efficiency and position-effe...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Kim, Jongrae, Lee, Sangmuk, Baek, Kwangryul, Jin, EonSeon
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7099044/
https://ncbi.nlm.nih.gov/pubmed/32265959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2020.00306
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