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The strengths and limitations of using biolayer interferometry to monitor equilibrium titrations of biomolecules
Every method used to quantify biomolecular interactions has its own strengths and limitations. To quantify protein‐DNA binding affinities, nitrocellulose filter binding assays with (32)P‐labeled DNA quantify K (d) values from 10(−12) to 10(−8) M but have several technical limitations. Here, we consi...
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| Publicado en: | Protein Sci |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
John Wiley & Sons, Inc.
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7096710/ https://ncbi.nlm.nih.gov/pubmed/31943488 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3827 |
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