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A surface plasmon resonance-based assay for small molecule inhibitors of human cyclophilin A

A simple protocol for generating a highly stable and active surface plasmon resonance (SPR) sensor surface of recombinant human hexahistidine cyclophilin A (His-CypA) is described. The sensor surface was sensitive and stable enough to allow, for the first time, the screening and ranking of several n...

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Vydáno v:Anal Biochem
Hlavní autoři: Wear, Martin A., Patterson, Alan, Malone, Kirk, Dunsmore, Colin, Turner, Nicholas J., Walkinshaw, Malcolm D.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier Inc. 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7094681/
https://ncbi.nlm.nih.gov/pubmed/16102717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2005.06.037
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