A secretary bi-cistronic baculovirus expression system with improved production of the HA1 protein of H6 influenza virus in insect cells and Spodoptera litura larvae

A bi-cistronic baculovirus expression vector was constructed to facilitate the expression, detection, and isolation of the hemagglutinin (HA) fragment HA1 of H6N1 avian influenza virus (AIV) in an insect and a culture of its cells. In this construct, the GP67sp signal peptide promoted the secretion...

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Publicado en:J Immunol Methods
Autores principales: Hsieh, Ming-Shou, He, Jie-Long, Wu, Tzong-Yuan, Juang, Rong-Huay
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier B.V. 2018
Materias:
Research Paper
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7094261/
https://ncbi.nlm.nih.gov/pubmed/29894745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2018.06.001
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