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Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input

We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Daigneault, Bradford W, Rajput, Sandeep K, Smith, George W
Formato: Artigo
Idioma:Inglês
Publicado em: Future Science Ltd 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7092705/
https://ncbi.nlm.nih.gov/pubmed/31937114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2019-0121
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