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Simple workflow for genome and methylation analyses of ejaculated bovine spermatozoa with low sperm input

We developed a simplified workflow of gDNA extraction from ejaculated bovine sperm using a low total number of sperm and a short time frame that yields high-quality DNA suitable for downstream methylation and genome analyses. These techniques have broad implications in human biomedical sciences and...

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Bibliografische gegevens
Gepubliceerd in:Biotechniques
Hoofdauteurs: Daigneault, Bradford W, Rajput, Sandeep K, Smith, George W
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Future Science Ltd 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7092705/
https://ncbi.nlm.nih.gov/pubmed/31937114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2019-0121
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