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Engineering, delivery, and biological validation of artificial microRNA clusters for gene therapy applications

The cellular machinery regulating microRNA biogenesis and maturation relies on a small number of simple steps and minimal biological requirements, and is broadly conserved in all eukaryotic cells. The same holds true in disease. This allows for a significant degree of freedom in the engineering of t...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Bhaskaran, Vivek, Yao, Yizheng, Bei, Fengfeng, Peruzzi, Pierpaolo
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7089775/
https://ncbi.nlm.nih.gov/pubmed/31748752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0241-8
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