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Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus

We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Japanese encephalitis virus (JEV). The sensitivity of the JEV RT-LAMP assay was in concordance with that of real-time RT-PCR and 10-fold more sensitive than that of conventional RT...

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Bibliografiske detaljer
Udgivet i:Mol Biol Rep
Main Authors: Chen, Zhiyong, Liao, Yuxue, Ke, Xuemei, Zhou, Jie, Chen, Yixiong, Gao, LuLu, Chen, Qing, Yu, Shouyi
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7089266/
https://ncbi.nlm.nih.gov/pubmed/21116858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11033-010-0525-0
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