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Synthesis in Escherichia coli cells and characterization of the active exoribonuclease of severe acute respiratory syndrome coronavirus

The nsp14 protein, an exoribonuclease of the DEDD superfamily encoded by severe acute respiratory syndrome coronavirus (SARS-CoV), was expressed in fusion with different affinity tags. The recombinant nsp14 proteins with either GST fusion or 6-histidine tag were shown to possess ribonuclease activit...

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Detalhes bibliográficos
Publicado no:Mol Biol
Main Authors: Chen, P., Hu, T., Jiang, M., Guo, D.
Formato: Artigo
Idioma:Inglês
Publicado em: SP MAIK Nauka/Interperiodica 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7089200/
https://ncbi.nlm.nih.gov/pubmed/32214468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1134/S0026893309030091
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