Application of a Real-Time Polymerase Chain Reaction with Internal Positive Control for Detection and Quantification of Enterovirus in Cerebrospinal Fluid

A quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method based on TaqMan technology was developed to determine the presence and amount of enterovirus RNA. In order to prevent false-negative results, a one-step multiplex RT-PCR was optimized. It contains two dual-label...

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Publicado en:Eur J Clin Microbiol Infect Dis
Autores principales: Monpoeho, S., Coste-Burel, M., Costa-Mattioli, M., Besse, B., Chomel, J., Billaudel, S., Ferré, V.
Formato: Artigo
Lenguaje:Inglês
Publicado: Springer-Verlag 2002
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7088111/
https://ncbi.nlm.nih.gov/pubmed/12172744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10096-002-0766-5
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