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Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death k...

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Bibliografiske detaljer
Udgivet i:Biotechnol Lett
Main Authors: Lai, Chia-Wei, Chung, Yao-Chi, Lai, Yiu-Kay, Chang, Margaret Dah-Tsyr, Hu, Yu-Chen
Format: Artigo
Sprog:Inglês
Udgivet: Kluwer Academic Publishers 2005
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7087856/
https://ncbi.nlm.nih.gov/pubmed/16091881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-005-7176-6
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