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Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductases from higher plants
Messenger RNAs encoding the nitrate reductase apoenzyme from tobacco can be translated in a cell-free system. Poly(A)(+) mRNA fractions from the 23-32 S area of a sucrose gradient were used to build a cDNA library in the expression vector λgt11 with an efficiency of cloning of approximately 10(4) re...
Shranjeno v:
| izdano v: | Mol Gen Genet |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Springer-Verlag
1987
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7087718/ https://ncbi.nlm.nih.gov/pubmed/17193712 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/BF00331162 |
| Oznake: |
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