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Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein

The complete nonstructural NS5 gene of Japanese encephalitis virus (JEV) was amplified and cloned into an expression vector. The NS5 protein was expressed in Escherichia coli and purified by His-tag based affinity chromatography. This recombinant NS5 protein exhibited RNA-dependent RNA polymerase (R...

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Bibliografiske detaljer
Udgivet i:Arch Virol
Main Authors: Yu, F., Hasebe, F., Inoue, S., Mathenge, E. G. M., Morita, K.
Format: Artigo
Sprog:Inglês
Udgivet: Springer-Verlag 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7086876/
https://ncbi.nlm.nih.gov/pubmed/17577613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00705-007-1007-0
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