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Visualizing the Synaptic and Cellular Ultrastructure in Neurons Differentiated from Human Induced Neural Stem Cells—An Optimized Protocol

The size of the synaptic subcomponents falls below the limits of visible light microscopy. Despite new developments in advanced microscopy techniques, the resolution of transmission electron microscopy (TEM) remains unsurpassed. The requirements of tissue preservation are very high, and human post m...

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Détails bibliographiques
Publié dans:Int J Mol Sci
Auteurs principaux: Capetian, Philipp, Müller, Lorenz, Volkmann, Jens, Heckmann, Manfred, Ergün, Süleyman, Wagner, Nicole
Format: Artigo
Langue:Inglês
Publié: MDPI 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7084184/
https://ncbi.nlm.nih.gov/pubmed/32131546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21051708
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