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Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function
A recent genome-wide screen identified ~300 essential or growth-supporting genes in the dental caries pathogen Streptococcus mutans. To be able to study these genes, we built a CRISPR interference tool around the Cas9 nuclease (Cas9(Smu)) encoded in the S. mutans UA159 genome. Using a xylose-inducib...
में बचाया:
| में प्रकाशित: | PLoS Pathog |
|---|---|
| मुख्य लेखकों: | , , , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Public Library of Science
2020
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7082069/ https://ncbi.nlm.nih.gov/pubmed/32150575 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.ppat.1008344 |
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