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Optimization of small RNA library preparation protocol from human urinary exosomes

BACKGROUND: Sequencing of miRNAs isolated from exosomes has great potential to identify novel disease biomarkers, but exosomes have low amount of RNA, hindering adequate analysis and quantification. Here, we have assessed several steps in developing an optimized small RNA (sRNA) library preparation...

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Библиографические подробности
Опубликовано в: :J Transl Med
Главные авторы: Olivares, Dolores, Perez-Hernandez, Javier, Perez-Gil, Daniel, Chaves, Felipe J., Redon, Josep, Cortes, Raquel
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2020
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7081560/
https://ncbi.nlm.nih.gov/pubmed/32188466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12967-020-02298-9
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