Optimization of small RNA library preparation protocol from human urinary exosomes

BACKGROUND: Sequencing of miRNAs isolated from exosomes has great potential to identify novel disease biomarkers, but exosomes have low amount of RNA, hindering adequate analysis and quantification. Here, we have assessed several steps in developing an optimized small RNA (sRNA) library preparation...

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Bibliografiske detaljer
Udgivet i:J Transl Med
Main Authors: Olivares, Dolores, Perez-Hernandez, Javier, Perez-Gil, Daniel, Chaves, Felipe J., Redon, Josep, Cortes, Raquel
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2020
Fag:
Methodology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7081560/
https://ncbi.nlm.nih.gov/pubmed/32188466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12967-020-02298-9
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