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Optimization of small RNA library preparation protocol from human urinary exosomes
BACKGROUND: Sequencing of miRNAs isolated from exosomes has great potential to identify novel disease biomarkers, but exosomes have low amount of RNA, hindering adequate analysis and quantification. Here, we have assessed several steps in developing an optimized small RNA (sRNA) library preparation...
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| Publicado no: | J Transl Med |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7081560/ https://ncbi.nlm.nih.gov/pubmed/32188466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12967-020-02298-9 |
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