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Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis
[Image: see text] The pH dependence of the trypsin-catalyzed hydrolysis of N-α-benzyloxycarbonyl-l-lysine p-nitroanilide has been studied at 25 °C. k(cat)/K(M) was maximal at alkaline pH values but decreased with decreasing pH. k(cat)/K(M) was dependent on free enzyme pK(a) values of 6.75 ± 0.09 and...
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| Yayımlandı: | ACS Omega |
|---|---|
| Yazar: | |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
American Chemical Society
2020
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| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7081292/ https://ncbi.nlm.nih.gov/pubmed/32201777 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.9b03750 |
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