Improving the Thermostability of Rhizopus chinensis Lipase Through Site-Directed Mutagenesis Based on B-Factor Analysis

In order to improve the thermostability of lipases derived from Rhizopus chinensis, we identified lipase (Lipr27RCL) mutagenesis sites that were associated with enhanced flexibility based upon B-factor analysis and multiple sequence alignment. We found that two mutated isoforms (Lipr27RCL-K64N and L...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Front Microbiol
Prif Awduron: Jiang, Zhanbao, Zhang, Chengbo, Tang, Minyuan, Xu, Bo, Wang, Lili, Qian, Wen, He, Jiandong, Zhao, Zhihong, Wu, Qian, Mu, Yuelin, Ding, Junmei, Zhang, Rui, Huang, Zunxi, Han, Nanyu
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Frontiers Media S.A. 2020
Pynciau:
Microbiology
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7063977/
https://ncbi.nlm.nih.gov/pubmed/32194535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2020.00346
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