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NMR-Based Activity Assays for Determining Compound Inhibition, IC(50) Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
NMR spectroscopy is often used for the identification and characterization of enzyme inhibitors in drug discovery, particularly in the context of fragment screening. NMR-based activity assays are ideally suited to work at the higher concentrations of test compounds required to detect these weaker in...
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| 發表在: | J Vis Exp |
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| Main Authors: | , , , , , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2019
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7061360/ https://ncbi.nlm.nih.gov/pubmed/31305530 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59928 |
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