Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads

The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the objective of check if this can alter the biocatal...

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Detalhes bibliográficos
Publicado no:Front Bioeng Biotechnol
Main Authors: Arana-Peña, Sara, Rios, Nathalia S., Carballares, Diego, Mendez-Sanchez, Carmen, Lokha, Yuliya, Gonçalves, Luciana R. B., Fernandez-Lafuente, Roberto
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Bioengineering and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7059646/
https://ncbi.nlm.nih.gov/pubmed/32181245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.00036
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