Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) for Metabolome and Proteome Analyses

The isolation of organelles facilitates the focused analysis of subcellular protein and metabolite pools. Here we present a technique for the affinity purification of plant mitochondria (Mito-AP). The stable ectopic expression of a mitochondrial outer membrane protein fused to a GFP:Strep tag in Ara...

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Bibliographic Details
Published in:Plant Physiol
Main Authors: Niehaus, Markus, Straube, Henryk, Künzler, Patrick, Rugen, Nils, Hegermann, Jan, Giavalisco, Patrick, Eubel, Holger, Witte, Claus-Peter, Herde, Marco
Format: Artigo
Language:Inglês
Published: American Society of Plant Biologists 2020
Subjects:
Breakthrough Technologies
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7054873/
https://ncbi.nlm.nih.gov/pubmed/31911558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.19.00736
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