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In vitro ribosome synthesis and evolution through ribosome display

Directed evolution of the ribosome for expanded substrate incorporation and novel functions is challenging because the requirement of cell viability limits the mutations that can be made. Here we address this challenge by combining cell-free synthesis and assembly of translationally competent riboso...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Hammerling, Michael J., Fritz, Brian R., Yoesep, Danielle J., Kim, Do Soon, Carlson, Erik D., Jewett, Michael C.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7048773/
https://ncbi.nlm.nih.gov/pubmed/32111839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-14705-2
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