Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance

Metagenomic next-generation sequencing (mNGS), the shotgun sequencing of RNA and DNA from clinical samples, has proved useful for broad-spectrum pathogen detection and the genomic surveillance of viral outbreaks. An additional target enrichment step is generally needed for high-sensitivity pathogen...

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Publicado no:Nat Microbiol
Main Authors: Deng, Xianding, Achari, Asmeeta, Federman, Scot, Yu, Guixia, Somasekar, Sneha, Bártolo, Inês, Yagi, Shigeo, Mbala-Kingebeni, Placide, Kapetshi, Jimmy, Ahuka-Mundeke, Steve, Muyembe-Tamfum, Jean-Jacques, Ahmed, Asim A., Ganesh, Vijay, Tamhankar, Manasi, Patterson, Jean L., Ndembi, Nicaise, Mbanya, Dora, Kaptue, Lazare, McArthur, Carole, Muñoz-Medina, José E., Gonzalez-Bonilla, Cesar R., López, Susana, Arias, Carlos F., Arevalo, Shaun, Miller, Steve, Stone, Mars, Busch, Michael, Hsieh, Kristina, Messenger, Sharon, Wadford, Debra A., Rodgers, Mary, Cloherty, Gavin, Faria, Nuno R., Thézé, Julien, Pybus, Oliver G., Neto, Zoraima, Morais, Joana, Taveira, Nuno, R. Hackett, John, Chiu, Charles Y.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7047537/
https://ncbi.nlm.nih.gov/pubmed/31932713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41564-019-0637-9
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