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Reduction in the structural instability of cloned eukaryotic tandem-repeat DNA by low-temperature culturing of host bacteria

For accurate analyses of eukaryotic tandem-repeat DNA, it is often required to clone a genomic DNA fragment into a bacterial plasmid. It is, however, a serious problem that tandem-repeat DNA is frequently subjected to structural changes during maintenance or amplification in the host bacteria. Here,...

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Bibliografiske detaljer
Udgivet i:Genet Res (Camb)
Main Authors: THAPANA, WATCHARAPORN, SUJIWATTANARAT, PENPORN, SRIKULNATH, KORNSORN, HIRAI, HIROHISA, KOGA, AKIHIKO
Format: Artigo
Sprog:Inglês
Udgivet: Cambridge University Press 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7045003/
https://ncbi.nlm.nih.gov/pubmed/25578068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1017/S0016672314000172
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