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Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry

Enzymatic digestion for protein sequencing usually requires much time, and does not always result in high sequence coverage. Here we report the use of aqueous microdroplets to accelerate enzymatic reactions and, in particular, to improve protein sequencing. When a room temperature aqueous solution c...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nat Commun
Prif Awduron: Zhong, Xiaoqin, Chen, Hao, Zare, Richard N.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Nature Publishing Group UK 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7044307/
https://ncbi.nlm.nih.gov/pubmed/32103000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-14877-x
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