Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking

Ligation-mediated PCR (LM-PCR) is a classical method for isolating flanking sequences; however, it has a common limitation of reduced success rate owing to the circularization or multimerization of target restriction fragments including the known sequence. To address this limitation, we developed a...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Yu, Dong, Zhou, Tianshun, Sun, Xuewu, Sun, Zhizhong, Sheng, Xiabing, Tan, Yanning, Liu, Ling, Ouyang, Ning, Xu, Ke, Shi, Kaibing, Yuan, Guilong, Ding, Jia, Duan, Meijuan, Yuan, Dingyang
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2020
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7044209/
https://ncbi.nlm.nih.gov/pubmed/32103092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-60411-w
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