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Tendentious effects of automated and manual metagenomic DNA purification protocols on broiler gut microbiome taxonomic profiling

Here, we developed protocols to improve sensitivity, rigor and comparability of 16S rRNA gene amplification-based next-generation sequencing (NGS) results. A thorough study was performed by evaluating extraction efficiency with respect to the yield, purity, fragmentation of the purified DNA, and seq...

詳細記述

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書誌詳細
出版年:Sci Rep
主要な著者: Fidler, Gabor, Tolnai, Emese, Stagel, Aniko, Remenyik, Judit, Stundl, Laszlo, Gal, Ferenc, Biro, Sandor, Paholcsek, Melinda
フォーマット: Artigo
言語:Inglês
出版事項: Nature Publishing Group UK 2020
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7042355/
https://ncbi.nlm.nih.gov/pubmed/32099013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-60304-y
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