Tendentious effects of automated and manual metagenomic DNA purification protocols on broiler gut microbiome taxonomic profiling

Here, we developed protocols to improve sensitivity, rigor and comparability of 16S rRNA gene amplification-based next-generation sequencing (NGS) results. A thorough study was performed by evaluating extraction efficiency with respect to the yield, purity, fragmentation of the purified DNA, and seq...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Fidler, Gabor, Tolnai, Emese, Stagel, Aniko, Remenyik, Judit, Stundl, Laszlo, Gal, Ferenc, Biro, Sandor, Paholcsek, Melinda
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7042355/
https://ncbi.nlm.nih.gov/pubmed/32099013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-60304-y
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