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人骨形态发生蛋白2真核表达载体构建及其基因/壳聚糖纳米复合体制备
OBJECTIVE: To clone and construct a eukaryotic expression vector of human bone morphogenetic protein (BMP) 2 and histidine in vitro and synthesize chitosan (CS)/pIRES2-EGFP-hBMP2-His nanoparticles. METHODS: pMD18T-hBMP2-His was digested by EcoRⅠand BamHⅠto obtain the hBMP2-His gene, which was insert...
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| Publicado no: | Hua Xi Kou Qiang Yi Xue Za Zhi |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
华西口腔医学杂志编辑部
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7041026/ https://ncbi.nlm.nih.gov/pubmed/25490830 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7518/hxkq.2014.05.016 |
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