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Microinjection of dsRNA in tardigrades

Classical genetic analysis in Hypsibius exemplaris is a challenge because these animals are parthenogens. The publication of the H. exemplaris genome has facilitated the study of targeted genes by RNA interference (RNAi), a robust mechanism to disrupt gene function. This protocol describes microinje...

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Bibliographic Details
Published in:Cold Spring Harb Protoc
Main Author: Tenlen, Jennifer R.
Format: Artigo
Language:Inglês
Published: 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7038633/
https://ncbi.nlm.nih.gov/pubmed/30385674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot102368
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