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Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

BACKGROUND: The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are in widespread use. These assays are predominantly singleplex; i.e. one assay tests for the presenc...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Player, Robert, Verratti, Kathleen, Staab, Andrea, Bradburne, Christopher, Grady, Sarah, Goodwin, Bruce, Sozhamannan, Shanmuga
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7026984/
https://ncbi.nlm.nih.gov/pubmed/32066372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-020-6557-5
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