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In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli

The in-cell NMR technique offers significant insights into the structure and function of heterologous proteins in the physiological intracellular environment at an atomic resolution. Escherichia coli (E. coli) is one of the most widely used host cells for heterologous protein expression in structura...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Sugiki, Toshihiko, Yamaguchi, Yoshihiro, Fujiwara, Toshimichi, Inouye, Masayori, Ito, Yutaka, Kojima, Chojiro
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7015911/
https://ncbi.nlm.nih.gov/pubmed/32051433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-59076-2
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