Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

BACKGROUND: Polymerase chain reaction (PCR) analysis using DNA from dried blood spot (DBS) samples on filter paper is a critical technique for spinal muscular atrophy (SMA) newborn screening. However, DNA extraction from DBS is time-consuming, and elimination of PCR inhibitors from DBS is almost imp...

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Veröffentlicht in:Kobe J Med Sci
Hauptverfasser: TAKEUCHI, ATSUKO, TODE, CHISATO, NISHINO, MASAYOSHI, WIJAYA, YOGIK ONKY SILVANA, NIBA, EMMA TABE EKO, AWANO, HIROYUKI, TAKESHIMA, YASUHIRO, SAITO, TOSHIO, SAITO, KAYOKO, LAI, POH SAN, BOUIKE, YOSHIHIRO, NISHIO, HISAHIDE, SHINOHARA, MASAKAZU
Format: Artigo
Sprache:Inglês
Veröffentlicht: Kobe University School Of Medicine 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7012323/
https://ncbi.nlm.nih.gov/pubmed/32029694
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