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Demonstration of flat-top beam illumination in widefield multiphoton microscopy

Multiphoton microscopy provides a suitable technique for imaging biological tissues with submicrometer resolution. Usually a Gaussian beam (GB) is used for illumination, leading to a reduced power efficiency in the multiphoton response and vignetting for a square-shaped imaging area. A flat-top beam...

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Detalhes bibliográficos
Publicado no:J Biomed Opt
Main Authors: Kabir, Mohammad M., Rajput, Hemangg S., Kelkar, Varun A., Salazar Coariti, Adriana C., Toussaint, Kimani C.
Formato: Artigo
Idioma:Inglês
Publicado em: Society of Photo-Optical Instrumentation Engineers 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7008505/
https://ncbi.nlm.nih.gov/pubmed/31729201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.25.1.014503
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