Functional dynamics of a single tryptophan residue in a BLUF protein revealed by fluorescence spectroscopy

Blue Light Using Flavin (BLUF) domains are increasingly being adopted for use in optogenetic constructs. Despite this, much remains to be resolved on the mechanism of their activation. The advent of unnatural amino acid mutagenesis opens up a new toolbox for the study of protein structural dynamics....

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Publicat a:Sci Rep
Autors principals: Karadi, Kristof, Kapetanaki, Sofia M., Raics, Katalin, Pecsi, Ildiko, Kapronczai, Robert, Fekete, Zsuzsanna, Iuliano, James N., Collado, Jinnette Tolentino, Gil, Agnieszka A., Orban, Jozsef, Nyitrai, Miklos, Greetham, Greg M., Vos, Marten H., Tonge, Peter J., Meech, Stephen R., Lukacs, Andras
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2020
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7005313/
https://ncbi.nlm.nih.gov/pubmed/32029866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-59073-5
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