Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability

This study aimed to compare the quality of human spermatozoa vitrified by direct plunging into liquid nitrogen vs. liquid air. Spermatozoa were divided into three groups: fresh spermatozoa (Group F) were used as a control. Spermatozoa suspension (20 μl) was vitrified in open granules by direct dropp...

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Bibliographic Details
Published in:Biomed Res Int
Main Authors: Wang, Mengying, Isachenko, Evgenia, Todorov, Plamen, Rahimi, Gohar, Mallmann, Peter, Katkov, Igor I., Isachenko, Vladimir
Format: Artigo
Language:Inglês
Published: Hindawi 2020
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7003260/
https://ncbi.nlm.nih.gov/pubmed/32047807
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2020/2934315
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