Three-Dimensional Visualization of APEX2-Tagged Erg11 in Saccharomyces cerevisiae Using Focused Ion Beam Scanning Electron Microscopy

The determination of the exact location of a protein in the cell is essential to the understanding of biological processes. Here, we report for the first time the visualization of a protein of interest in Saccharomyces cerevisiae using focused ion beam scanning electron microscopy (FIB-SEM). As a pr...

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Detalhes bibliográficos
Publicado no:mSphere
Main Authors: Kerstens, Winnie, Kremer, Anneke, Holtappels, Michelle, Borghgraef, Peter, Lippens, Saskia, Van Dijck, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2020
Assuntos:
Resource Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7002314/
https://ncbi.nlm.nih.gov/pubmed/32024705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00981-19
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