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Genetic Suppression of Cryoprotectant Toxicity

We report here a new, unbiased forward genetic method that uses transposon-mediated mutagenesis to enable the identification of mutations that confer cryoprotectant toxicity resistance (CTR). Our method is to select for resistance to the toxic effects of M22, a much-studied whole-organ vitrification...

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Publicado en:Cryobiology
Autores principales: Cypser, James R., Chick, Wallace S., Fahy, Gregory M., Schumacher, Garrett J., Johnson, Thomas E.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2018
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7001869/
https://ncbi.nlm.nih.gov/pubmed/30458175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cryobiol.2018.11.003
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