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Weak cell cycle dependency but strong distortive effects of transfection with Lipofectamine 2000 in near‐physiologically synchronized cell culture

Previously, we reported a method to generate and validate cell cycle‐synchronized cultures of multiple mammalian suspension cell lines under near‐physiological conditions. This method was applied to elucidate the putative interdependencies of the cell cycle and recombinant protein expression in the...

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Vydáno v:Eng Life Sci
Hlavní autoři: Fuge, Grischa, Zeng, An‐Ping, Jandt, Uwe
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6999550/
https://ncbi.nlm.nih.gov/pubmed/32624780
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201600113
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